Composite

Part:BBa_K552003:Design

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-28)


CrtI Gene-Codon Optimized for Yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1746
    Illegal XbaI site found at 1527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1746
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1746
    Illegal BglII site found at 1323
    Illegal BglII site found at 1944
    Illegal BglII site found at 2103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1746
    Illegal XbaI site found at 1527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1746
    Illegal XbaI site found at 1527
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.


Source

The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm

References